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Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae)

Yıl 2020, Cilt: 8 Sayı: 4, 2289 - 2295, 29.10.2020

Ayşe Aygün Barış Gülcü

https://doi.org/10.29130/dubited.768800

Öz

In this study, Bacillus thrungiensis aizawai Bonnefoi & de Barjac, 1963 (Florbac), different isolates of Bacillus thuringiensis (Bt) kurstaki Berliner, 1915 (Biobit, Delfin, Rapax, Rebound) and spinosad (Spintor) were assessed for their efficacy against different instars of Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) under laboratory and field conditions in 2019 in Duzce Province, Turkey. Spinosad was the most effective bioinsecticide, followed by Bt aizawai and Bt kurstaki isolates against various instars of H. cunea. In the laboratory, at 96 hour post-treatment, spinosad caused 98% mortality in 2nd and 3rd instars, and 83% mortality in 4th and 5th instars; Bt aizawai caused 98% mortality in 2nd and 3rd instars and 43% mortality in 4th and 5th instars; and Bt kurstaki isolates caused 95% mortality in 2nd and 3rd instars and 51% mortality in 4th and 5th instars. The results from the field study showed similar trends. The older instars were less susceptible to all the tested bioinsecticides than the younger instars, which may be attributable to the phenomenon known as “maturation immunity”. Bacillus thuringiensis kurstaki has already been recommended for management of this pest. Whereas the data indicated that spinosad and Bt aizawai could also be used as bioinsecticides for control H. cunea larvae.

Anahtar Kelimeler

Bacillus thuringiensis spinosad Hyphantria cunea

Destekleyen Kurum

Düzce Üniversitesi Fen Bilimleri Enstitüsü

Teşekkür

We thank to Dr. Salih Tunc KAYA from Duzce University for his assistance in the analyses of data. We also thank to Dr. Derya ULUĞ from Adnan Menderes University, Aydın, Turkey and Dr. Harry K. KAYA from University of California, Davis, CA, USA for comments and advice on an earlier version of the manuscript. and for support of this study. This is a dissertation thesis in Institute of Science in Duzce University

Kaynakça

  • [1] T. D. Schowalter, D. R. Ring, “Biology and Management of the Fall Webworm, Hyphantria cunea (Lepidoptera: Erebidae),” Journal of Integrated Pest Management, vol. 8, pp. 1-6, 2017.
  • [2] C. Tuncer, İ. A. Kansu, “Konukçu bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)'ya etkileri üzerine araştırmalar,” Türk Entomoloji Dergisi, vol. 18, pp. 209-222, 1994.
  • [3] E. Akkuzu, T. Mol, “Amerikan beyaz kelebeği (Hyphantria cunea (Dry.)) üzerine biyolojik ve morfolojik araştırmalar,” Süleyman Demirel Üniversitesi Orman Fakültesi Dergisi, pp. 50-57, 2006.
  • [4] D. L. Wagner, Caterpillars of Eastern North America, Princeton University, Princeton, USA: Princeton University Press, 2005.
  • [5] Anonymous, “Bacillus thrungiensis aizawai strain abts-1857 aktif madde detay” [Online]. Available: https://bku.tarim.gov.tr/AktifMadde/Details/741. Accessed: 06.01.2018.
  • [6] T. Sparks C., G. D. Thompson, L. L. Larson, H. A. Kirst, O. K. Jantz, et al. “Biological characteristics of the spinosyns: a new naturally derived insect control agent”, Proceedings of the Beltwide Cotton Conference, San Antonio, Texas, USA, 1995, pp. 903-907.
  • [7] A. D. Jr. Oliver, “An Ecological Study of the Fall Webworm, Hyphantria cunea (Drury), in Louisiana”, Ph.D. dissertation, Louisiana State University, Louisiana, USA, 1963. [Online]. Avaible: https://digitalcommons.lsu.edu/gradschool_disstheses/820. Accessed: 12.06.2018.
  • [8] I. Saruhan, I. Akca, R. Kushiyev, “Toxicity of Some Biopesticides to the Fall Webworm, Hyphantria cunea Durry (Lepidoptera: Arctidae),” Egyptian Journal of Biological Pest Control, vol. 24, pp. 255-257, 2014.
  • [9] SPSS 22.0. Statistics for Windows (2013). IBM
  • [10] W. S. Abbott, “A method for computing the effectiveness of an insecticide,” Journal of Economic Entomology, vol. 18, pp. 265-267, 1925.
  • [11] J. Beuzelin, S. Brown, J. Davis, L. D. Foil, K. Healy, et al. Louisiana Insect Pest Management Guide, Baton Rouge, Los Angeles, USA, Louisiana State University Agricultural Center Press, 2016.
  • [12] G. B. Watson, “Actions of insecticidal spinosyns on γ-aminobutyric acid responses from small-diameter cockroach neurons,” Pesticide Biochemistry and Physiology, vol.71, pp. 20-28, 2001.
  • [13] O. Ecevit, C. Tuncer, G. Hatat, S. Keçeci, “İki farklı Bacillus thuringiensis preparatı (Thuricide ve Biobit) ile azinphos-methyl ve treflumuron'un Amerikan Beyaz Kelebeği (Hyphantria cunea Drury Lepidoptera: Arctiidae)'ne etkinliği üzerinde çalışmalar,” Türkiye 3. Biyolojik Mücadele Kongresi, İzmir, Türkiye, 1994, pp. 519-28.
  • [14] Y.Tanada, H. K. Kaya, Insect Pathology, San Diego, USA: Academic Press, 1993.

Spinosad ve Farklı Bacillus thuringiensis (Bacillaceae) Suşlarının Hyphantria cunea (Lepidoptera: Erebidae) Larvaları Üzerindeki Etkinliğinin Değerlendirilmesi

Yıl 2020, Cilt: 8 Sayı: 4, 2289 - 2295, 29.10.2020

Ayşe Aygün Barış Gülcü

https://doi.org/10.29130/dubited.768800

Öz

Bu çalışmada, Bacillus thrungiensis aizawai Bonnefoi & de Barjac, 1963 (Florbac), Bacillus thuringiensis (Bt) kurstaki Berliner, 1915 (Biobit, Delfin, Rapax, Rebound)’nin farklı ticari suşları ve spinosad (Spintor)’ın etkinliği Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae)’nın farklı larva dönemlerine karşı laboratuvar ve doğal koşullarda 2019 yılında Düzce ilinde test edilmiştir. Hyphantria cunea’nın farklı dönem larvalarına karşı en etkili biyoinsektisidin spinosad olduğu tespit edilmiştir. Spinosad’ı sırasıyla Bt aizawai ve Bt kurstaki suşları takip etmiştir. Laboratuvar deneylerinde 96 saat sonunda spinosad H. cunea’nın 2. ve 3. dönem larvalarında %98, 4. ve 5. dönem larvalarında ise %83 ölüm meydana getirmiştir. Bt aizawai 2. ve 3. dönem larvalarının %98’ini, 4. ve 5. dönem larvaların ise %43’ünü, Bt kurstaki suşları 2. ve 3. dönem larvaların ortalama %95’ini, 4. ve 5. dönem larvaların ise ortalama %51’ini öldürmüştür. Alan denemelerinde de spinosad en etkili sonuçları sergilemiştir. Çalışmada daha yaşlı larvaların daha az duyarlı oldukları görülmüştür. Bu durumun “maturation immunity” olarak bilinen olguyla bağlantılı olduğu düşünülmektedir. Bu zararlı ile mücadelede Bacillus thrungiensis (Bt) kurstaki zaten tavsiye edilmektedir. Bu çalışmada ise spinosad ve Bt aizawai etken maddesine sahip biyoinsektisitlerin de H. cunea’ya karşı kullanılabileceği gösterilmiştir.

Anahtar Kelimeler

Bacillus thuringiensis spinosad Hyphantria cunea

Kaynakça

  • [1] T. D. Schowalter, D. R. Ring, “Biology and Management of the Fall Webworm, Hyphantria cunea (Lepidoptera: Erebidae),” Journal of Integrated Pest Management, vol. 8, pp. 1-6, 2017.
  • [2] C. Tuncer, İ. A. Kansu, “Konukçu bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)'ya etkileri üzerine araştırmalar,” Türk Entomoloji Dergisi, vol. 18, pp. 209-222, 1994.
  • [3] E. Akkuzu, T. Mol, “Amerikan beyaz kelebeği (Hyphantria cunea (Dry.)) üzerine biyolojik ve morfolojik araştırmalar,” Süleyman Demirel Üniversitesi Orman Fakültesi Dergisi, pp. 50-57, 2006.
  • [4] D. L. Wagner, Caterpillars of Eastern North America, Princeton University, Princeton, USA: Princeton University Press, 2005.
  • [5] Anonymous, “Bacillus thrungiensis aizawai strain abts-1857 aktif madde detay” [Online]. Available: https://bku.tarim.gov.tr/AktifMadde/Details/741. Accessed: 06.01.2018.
  • [6] T. Sparks C., G. D. Thompson, L. L. Larson, H. A. Kirst, O. K. Jantz, et al. “Biological characteristics of the spinosyns: a new naturally derived insect control agent”, Proceedings of the Beltwide Cotton Conference, San Antonio, Texas, USA, 1995, pp. 903-907.
  • [7] A. D. Jr. Oliver, “An Ecological Study of the Fall Webworm, Hyphantria cunea (Drury), in Louisiana”, Ph.D. dissertation, Louisiana State University, Louisiana, USA, 1963. [Online]. Avaible: https://digitalcommons.lsu.edu/gradschool_disstheses/820. Accessed: 12.06.2018.
  • [8] I. Saruhan, I. Akca, R. Kushiyev, “Toxicity of Some Biopesticides to the Fall Webworm, Hyphantria cunea Durry (Lepidoptera: Arctidae),” Egyptian Journal of Biological Pest Control, vol. 24, pp. 255-257, 2014.
  • [9] SPSS 22.0. Statistics for Windows (2013). IBM
  • [10] W. S. Abbott, “A method for computing the effectiveness of an insecticide,” Journal of Economic Entomology, vol. 18, pp. 265-267, 1925.
  • [11] J. Beuzelin, S. Brown, J. Davis, L. D. Foil, K. Healy, et al. Louisiana Insect Pest Management Guide, Baton Rouge, Los Angeles, USA, Louisiana State University Agricultural Center Press, 2016.
  • [12] G. B. Watson, “Actions of insecticidal spinosyns on γ-aminobutyric acid responses from small-diameter cockroach neurons,” Pesticide Biochemistry and Physiology, vol.71, pp. 20-28, 2001.
  • [13] O. Ecevit, C. Tuncer, G. Hatat, S. Keçeci, “İki farklı Bacillus thuringiensis preparatı (Thuricide ve Biobit) ile azinphos-methyl ve treflumuron'un Amerikan Beyaz Kelebeği (Hyphantria cunea Drury Lepidoptera: Arctiidae)'ne etkinliği üzerinde çalışmalar,” Türkiye 3. Biyolojik Mücadele Kongresi, İzmir, Türkiye, 1994, pp. 519-28.
  • [14] Y.Tanada, H. K. Kaya, Insect Pathology, San Diego, USA: Academic Press, 1993.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Ayşe Aygün Bu kişi benim 0000-0001-6718-0382

Barış Gülcü 0000-0002-4808-1538

Yayımlanma Tarihi 29 Ekim 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 8 Sayı: 4

Kaynak Göster

APA Aygün, A., & Gülcü, B. (2020). Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae). Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 8(4), 2289-2295. https://doi.org/10.29130/dubited.768800
AMA Aygün A, Gülcü B. Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae). DÜBİTED. Ekim 2020;8(4):2289-2295. doi:10.29130/dubited.768800
Chicago Aygün, Ayşe, ve Barış Gülcü. “Evaluation of Spinosad and Different Bacillus Thuringiensis (Bacillaceae) Strains Against the Larvae of Hyphantria Cunea (Lepidoptera: Erebidae)”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 8, sy. 4 (Ekim 2020): 2289-95. https://doi.org/10.29130/dubited.768800.
EndNote Aygün A, Gülcü B (01 Ekim 2020) Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae). Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8 4 2289–2295.
IEEE A. Aygün ve B. Gülcü, “Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae)”, DÜBİTED, c. 8, sy. 4, ss. 2289–2295, 2020, doi: 10.29130/dubited.768800.
ISNAD Aygün, Ayşe - Gülcü, Barış. “Evaluation of Spinosad and Different Bacillus Thuringiensis (Bacillaceae) Strains Against the Larvae of Hyphantria Cunea (Lepidoptera: Erebidae)”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 8/4 (Ekim 2020), 2289-2295. https://doi.org/10.29130/dubited.768800.
JAMA Aygün A, Gülcü B. Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae). DÜBİTED. 2020;8:2289–2295.
MLA Aygün, Ayşe ve Barış Gülcü. “Evaluation of Spinosad and Different Bacillus Thuringiensis (Bacillaceae) Strains Against the Larvae of Hyphantria Cunea (Lepidoptera: Erebidae)”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, c. 8, sy. 4, 2020, ss. 2289-95, doi:10.29130/dubited.768800.
Vancouver Aygün A, Gülcü B. Evaluation of Spinosad and Different Bacillus thuringiensis (Bacillaceae) Strains against the Larvae of Hyphantria cunea (Lepidoptera: Erebidae). DÜBİTED. 2020;8(4):2289-95.
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