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Year 2023, Volume: 6 Issue: 2, 304 - 307, 31.08.2023

Alper Togay Duygu Tekin Şeyma Ecem Irmak Utku Ercan Nisel Yılmaz

https://doi.org/10.36516/jocass.1342678

Abstract

References

  • 1.Nordmann P, Dortet L, Poirel L. Carbapenem resistance in Enterobacteri¬aceae: here is the storm. Trends Mol Med. 2012; 18(5): 263-72. https://doi.org/10.1016/j.molmed.2012.03.003
  • 2.Lewis CM, Zervos MJ. Clinical manifestations of enterococcal infection. Eur J Clin Microbiol Infect Dis. 1990; 9(2): 111-7. https://doi.org/10.1007/BF01963635
  • 3.Çetinkaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clin Mi¬crobiol Rev. 2000; 13(4): 686-707. https://doi.org/10.1128/CMR.13.4.686
  • 4.Joshi SG, Cooper M, Yost A, et al. Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and mem¬brane lipid peroxidation in Escherichia coli. Antimicrob Agents Chemother. 2011; 55(3): 1053-62. https://doi.org/10.1128/AAC.01002-10
  • 5.Ercan UK, Wang H, Ji H, et al. Nonequilibrium Plasma-activated antimi-crobial solutions are broad-spectrum and retain their efficacies for ex-tended period of time. Plasma Processes Polym. 2013; 10(6): 544-55. https://doi.org/10.1002/ppap.201200104
  • 6.Fridman G, Friedman G, Gutsol A, et al. Applied plasma medicine. Plasma Process Polym. 2008; 5(6): 503-33. https://doi.org/10.1002/ppap.200700154
  • 7.Oztan MO, Ercan UK, Aksoy Gokmen A, et al. Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces micro¬bial load in rat acute peritonitis model. Sci Rep. 2022; 12(1): 3646. https://doi.org/10.1038/s41598-022-07598-2
  • 8.Lagadinou M, Onisor MO, Rigas A, et al. Antimicrobial Properties on Non-An¬tibiotic Drugs in the Era of Increased Bacterial Resistance. Antibiotics. 2020; 9(3): 107. https://doi.org/10.3390/antibiotics9030107
  • 9.World Health Organization. "Antimicrobial resistance surveillance in Eu¬rope 2022-2020 data." (2022). https://apps.who.int/iris/handle/10665/351141
  • 10.Konwar AN, Hazarika SN, Bharadwaj P, et al. Emerging Non-Traditional Ap¬proaches to Combat Antibiotic Resistance. Curr Microbiol. 2022; 79(11): 330. https://doi.org/10.1007/s00284-022-03029-7
  • 11.Ercan UK, Sen B, Brooks AD, et al. Escherichia coli cellular responses to ex¬posure to atmospheric-pressure dielectric barrier discharge plasma-treated N-acetylcysteine solution. J Appl Microbiol. 2018; 125(2): 383-97. https://doi.org/10.1111/jam.13777
  • 12.Friedman G, Gutsol A, Shekhter AB, et al. Applied plasma medicine. Plasma Process Polym. 2008; 5(6): 503-33. https://doi.org/10.1002/ppap.200700154
  • 13.Graves DB. The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J Phys D Appl Phys. 2012; 45: 263001. https://doi.org/10.1088/0022-3727/45/26/263001
  • 14.Laroussi M, Leipold F. Evaluation of the roles of reactive species, heat, and UV radiation in the inactivation of bacterial cells by air plasmas at at-mospheric pressure. Int J Mass Spectrom. 2004; 233: 81-6. https://doi.org/10.1016/j.ijms.2003.11.016
  • 15.Machala Z, Tarabova B, Hensel K, et al. Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bacteri¬cidal Effects. Plasma Process Polym. 2013;10:649-59. https://doi.org/10.1002/ppap.201200113
  • 16.Ercan UK, Smith J, Ji HF, et al. Chemical Changes in Nonthermal Plasma-Treated N-Acetylcysteine (NAC) Solution and Their co ntribution to Bacte¬rial Inactivation Sci Rep. 2016; 6: 20365. https://doi.org/10.1038/srep20365
  • 17.Han L, Patil S, Boehm D, et al. Mechanisms of Inactivation by High-Volt¬age Atmospheric Cold Plasma Differ for Escherichia coli and Staphylococ¬cus au¬reus. Appl Environ Microbiol. 2015; 82(2): 450-8. https://doi.org/10.1128/AEM.02660-15

Investigation of the Efficacy Results of Atmospheric Cold Plasma Against Multi-Resistant Bacterial Strains

Year 2023, Volume: 6 Issue: 2, 304 - 307, 31.08.2023

Alper Togay Duygu Tekin Şeyma Ecem Irmak Utku Ercan Nisel Yılmaz

https://doi.org/10.36516/jocass.1342678

Abstract

Giriş: Amacımız Atmosferik Soğuk Plazma ile muamele edilmiş PBS'nin Gram pozitif ve Gram negatif çoklu ilaca dirençli bakterilere karşı etkinliğini araştırmaktır.
Gereç ve Yöntemler: Toplam 50 karbapenem dirençli Klebsiella pneumoniae ve 10 vankomisin dirençli Enterococcus faecium suşu çalışmaya dahil edilmiştir. 100 µl bakteri süspansiyonu (107 CFU/ml) üzerine 100 µl (1/2), 300 µl (3/4), 700 µl (7/8), 1500 µl (15/16), 3100 µl (31/32), 6300 µl ACP ile muamele edilmiş PBS eklenmiştir. Süspansiyon pipetlendikten sonra oda sıcaklığında 30 dakika inkübe edilmiş, koyun kanlı agara ekilmiş ve 37°C'de bir gece (16-18 saat) inkübe edilmiştir.
Bulgular: Çalışılan tüm suşlar ASP ile muamele edilmiş PBS çözeltisi tarafından inhibe edilmiştir. Verilen dilüsyonlar bakteri çoğalmasının tamamen inhibe olduğu dilüsyonlardır. K. pneumoniae suşlarının 45'i ACP ile muamele edilmiş PBS çözeltisi ile 3/4 konsantrasyonda tamamen inhibe olurken, K. pneumoniae suşlarının 5'i ACP ile muamele edilmiş PBS çözeltisi ile 7/8 konsantrasyonda tamamen inhibe olmuştur. Vankomisine dirençli E. faecium suşları, K. pneumonia suşlarına göre daha yüksek miktarlarda plazma ile inhibe edilmiştir. E. faecium suşlarından üçü (15/16), E. faecium suşlarından üçü (31/32), E. faecium suşlarından dördü (63/64) ACP ile muamele edilmiş PBS solüsyonu tarafından tamamen inhibe edilmiştir.
Sonuç: ACP ile muamele edilmiş PBS çözeltisinin önemli antibiyotiklere dirençli hem Gram-pozitif hem de Gram-negatif bakterilere karşı etkili olduğu bulunmuştur. Seçilen Gram-negatif ve Gram-pozitif bakterilerde inaktivasyon için gereken ASP ile muamele edilmiş PBS konsantrasyonunda farklılıklar gözlenmiştir. Bununla birlikte, hem Gram-negatif hem de Gram-pozitif enfeksiyonlarda mevcut tedavi seçenekleri gün geçtikçe azaldığı için bu yöntem umut vericidir. ASP ile muamele edilmiş PBS sıvılarının bir tedavi yöntemi olarak kullanılması için ileri çalışmalara ihtiyaç vardır.

Keywords

plasma multidrug resistance efficacy anti-bacterial agents

References

  • 1.Nordmann P, Dortet L, Poirel L. Carbapenem resistance in Enterobacteri¬aceae: here is the storm. Trends Mol Med. 2012; 18(5): 263-72. https://doi.org/10.1016/j.molmed.2012.03.003
  • 2.Lewis CM, Zervos MJ. Clinical manifestations of enterococcal infection. Eur J Clin Microbiol Infect Dis. 1990; 9(2): 111-7. https://doi.org/10.1007/BF01963635
  • 3.Çetinkaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clin Mi¬crobiol Rev. 2000; 13(4): 686-707. https://doi.org/10.1128/CMR.13.4.686
  • 4.Joshi SG, Cooper M, Yost A, et al. Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and mem¬brane lipid peroxidation in Escherichia coli. Antimicrob Agents Chemother. 2011; 55(3): 1053-62. https://doi.org/10.1128/AAC.01002-10
  • 5.Ercan UK, Wang H, Ji H, et al. Nonequilibrium Plasma-activated antimi-crobial solutions are broad-spectrum and retain their efficacies for ex-tended period of time. Plasma Processes Polym. 2013; 10(6): 544-55. https://doi.org/10.1002/ppap.201200104
  • 6.Fridman G, Friedman G, Gutsol A, et al. Applied plasma medicine. Plasma Process Polym. 2008; 5(6): 503-33. https://doi.org/10.1002/ppap.200700154
  • 7.Oztan MO, Ercan UK, Aksoy Gokmen A, et al. Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces micro¬bial load in rat acute peritonitis model. Sci Rep. 2022; 12(1): 3646. https://doi.org/10.1038/s41598-022-07598-2
  • 8.Lagadinou M, Onisor MO, Rigas A, et al. Antimicrobial Properties on Non-An¬tibiotic Drugs in the Era of Increased Bacterial Resistance. Antibiotics. 2020; 9(3): 107. https://doi.org/10.3390/antibiotics9030107
  • 9.World Health Organization. "Antimicrobial resistance surveillance in Eu¬rope 2022-2020 data." (2022). https://apps.who.int/iris/handle/10665/351141
  • 10.Konwar AN, Hazarika SN, Bharadwaj P, et al. Emerging Non-Traditional Ap¬proaches to Combat Antibiotic Resistance. Curr Microbiol. 2022; 79(11): 330. https://doi.org/10.1007/s00284-022-03029-7
  • 11.Ercan UK, Sen B, Brooks AD, et al. Escherichia coli cellular responses to ex¬posure to atmospheric-pressure dielectric barrier discharge plasma-treated N-acetylcysteine solution. J Appl Microbiol. 2018; 125(2): 383-97. https://doi.org/10.1111/jam.13777
  • 12.Friedman G, Gutsol A, Shekhter AB, et al. Applied plasma medicine. Plasma Process Polym. 2008; 5(6): 503-33. https://doi.org/10.1002/ppap.200700154
  • 13.Graves DB. The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J Phys D Appl Phys. 2012; 45: 263001. https://doi.org/10.1088/0022-3727/45/26/263001
  • 14.Laroussi M, Leipold F. Evaluation of the roles of reactive species, heat, and UV radiation in the inactivation of bacterial cells by air plasmas at at-mospheric pressure. Int J Mass Spectrom. 2004; 233: 81-6. https://doi.org/10.1016/j.ijms.2003.11.016
  • 15.Machala Z, Tarabova B, Hensel K, et al. Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bacteri¬cidal Effects. Plasma Process Polym. 2013;10:649-59. https://doi.org/10.1002/ppap.201200113
  • 16.Ercan UK, Smith J, Ji HF, et al. Chemical Changes in Nonthermal Plasma-Treated N-Acetylcysteine (NAC) Solution and Their co ntribution to Bacte¬rial Inactivation Sci Rep. 2016; 6: 20365. https://doi.org/10.1038/srep20365
  • 17.Han L, Patil S, Boehm D, et al. Mechanisms of Inactivation by High-Volt¬age Atmospheric Cold Plasma Differ for Escherichia coli and Staphylococ¬cus au¬reus. Appl Environ Microbiol. 2015; 82(2): 450-8. https://doi.org/10.1128/AEM.02660-15
There are 17 citations in total.

Details

Primary Language English
Subjects Clinical Microbiology
Journal Section Articles
Authors

Alper Togay 0000-0002-5256-0064

Duygu Tekin 0000-0002-4913-622X

Şeyma Ecem Irmak 0009-0007-1673-8850

Utku Ercan 0000-0002-9762-2265

Nisel Yılmaz 0000-0001-7435-2461

Publication Date August 31, 2023
Acceptance Date August 22, 2023
Published in Issue Year 2023 Volume: 6 Issue: 2

Cite

APA Togay, A., Tekin, D., Irmak, Ş. E., Ercan, U., et al. (2023). Investigation of the Efficacy Results of Atmospheric Cold Plasma Against Multi-Resistant Bacterial Strains. Journal of Cukurova Anesthesia and Surgical Sciences, 6(2), 304-307. https://doi.org/10.36516/jocass.1342678
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