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PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM

Year 2024, Volume: 49 Issue: 3, 466 - 477

Bilge Sayın Börekçi Sedat Dönmez Ayşe Avcı

https://doi.org/10.15237/gida.GD24017

Abstract

In this study, alkaline protease enzyme production by a bacterial strain isolated from sludge samples collected from an anaerobic treatment system was investigated. According to the 16S rDNA sequence analysis, the isolate was identified as Thermoanaerobacter thermohydrosulfuricus (98.52%). Enzyme activity analyses revealed an optimum pH value of 10, an incubation time of 64 h, and a temperature of 35°C. Arabinose and casein hydrolysates were found to be the best carbon and nitrogen sources, respectively. Maximum protease activity was recorded (864.68 U/mL) when arabinose was used instead of glucose. Moreover, the addition of 1 g/L MgSO4.7H2O and 0.25 g/L Tween-80 to the medium increased the enzyme activity. Therefore, it can be concluded that T. thermohydrosulfuricus is a significant producer of alkaline protease enzymes in the culture medium. To the best of our knowledge, this is the first study to investigate the optimization of alkaline protease production by T. thermohydrosulfuricus.

Keywords

Extracellular proteolytic activity enzyme activity new isolate Thermoanaerobacter

References

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BELEDİYE ANAEROBİK ARITMA SİSTEMİNDEN İZOLE EDİLEN YENİ BİR ANAEROBİK BAKTERİ İLE ALKALİ PROTEAZ ÜRETİMİ

Year 2024, Volume: 49 Issue: 3, 466 - 477

Bilge Sayın Börekçi Sedat Dönmez Ayşe Avcı

https://doi.org/10.15237/gida.GD24017

Abstract

Bu çalışmada, anaerobik arıtma sisteminden toplanan çamur örneklerinden izole edilen bir bakteri suşunun alkali proteaz enzim üretimi araştırılmıştır. 16S rDNA dizi analizine göre, izolatın Thermoanaerobacter thermohidrosulfuricus (%98.52) olduğu tespit edilmiştir. Enzim aktivitesi analizleri, optimum pH değerinin 10, inkübasyon süresinin 64 saat ve sıcaklığın 35°C olduğunu ortaya çıkarmıştır. Arabinoz ve kazein hidrolizatlarının sırasıyla en iyi karbon ve nitrojen kaynakları olduğu bulunmuştur. Maksimum proteaz aktivitesi (864.68 U/mL) glikoz yerine arabinoz kullanıldığında kaydedilmiştir. Ayrıca, besiyerine 1 g/L MgSO4.7H2O ve 0.25 g/L Tween-80 ilavesi enzim aktivitesini artırmıştır. Bu nedenle, T. thermohidrosulfuricus’un kültür ortamında önemli bir alkali proteaz enzimi üreticisi olduğu sonucuna varılabilmektedir. Bildiğimiz kadarıyla bu çalışma, T. thermohidrosulfuricus’un alkali proteaz üretiminin optimizasyonunu araştıran ilk çalışmadır.

Keywords

Hücre dışı proteolitik aktivite enzim aktivitesi yeni izolat Thermoanaerobacter

References

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  • Akhter, K., Kiani, H. A., Ghous, T., Rasheed, A., Gillani, H., Akhtar, T. (2024). Production, partial purification and optimization of oilseed-based protease and its application as an efficient eco-friendly alternative for destaining and dehairing process. Waste and Biomass Valorization, 1-10. https://doi.org/10.1007/s12649-024-02438-y
  • Al-Dhabi, N. A., Esmail, G. A., Ghilan, A. K. M., Arasu, M. V., Duraipandiyan, V., Ponmurugan, K. (2020). Characterization and fermentation optimization of novel thermo stable alkaline protease from Streptomyces sp. Al-Dhabi-82 from the Saudi Arabian environment for eco-friendly and industrial applications. Journal of King Saud University-Science, 32(1),1258-1264. https://doi.org/10.1016/j.jksus.2019.11.011
  • Aqel, H., Al-Quadan, F., Yousef, T. K. (2012). A novel neutral protease from thermophilic Bacillus strain HUTBS62. Journal of Bioscience & Biotechnology, 1(2).
  • Arya, P. S., Yagnik, S. M., Rajput, K. N., Panchal, R. R., Raval, V. H. (2021). Understanding the basis of occurrence, biosynthesis, and implications of thermostable alkaline proteases. Applied Biochemistry and Biotechnology, 1-38. https://doi.org/10.1007/s12010-021-03701-x
  • Asha, B., Palaniswamy, M. (2018). Optimization of alkaline protease production by Bacillus cereus FT 1isolated from soil. Journal of Applied Pharmaceutical Science, 8(2), 119-127. https://doi.org/10.7324/JAPS.2018.8219
  • Banerjee, U. C., Sani, R. K., Azmi, W., Soni, R. (1999). Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive. Process Biochemistry, 35(1-2), 213-219. https://doi.org/10.1016/S0032-9592(99)00053-9
  • Bashir, F., Asgher, M., Hussain, F., Randhawa, M. A. (2018). Development and characterization of cross-linked enzyme aggregates of thermotolerant alkaline protease from Bacillus licheniformis. International Journal of Biological Macromolecules, 113, 944-951. https://doi.org/ 10.1016/j.ijbiomac.2018.03.009
  • Charles, P., Devanathan, V., Anbu, P., Ponnuswamy, M. N., Kalaichelvan, P. T., Hur, B. K. (2008). Purification, characterization and crystallization of an extracellular alkaline protease from Aspergillus nidulans HA‐10. Journal of Basic Microbiology, 48(5), 347-352. https://doi.org/ 10.1002/jobm.200800043
  • Chauhan, R. S., Mishra, R. M. (2020). Characterization of alkaline protease producing Bacillus halodurans RSCVS-PF21 isolated from poultry farm soil. Biosciences Biotechnology Research Asia, 17(2), 385-392. http://dx.doi.org/ 10.13005/bbra/2841
  • Chi, Z., Ma, C., Wang, P., Li, H. F. (2007). Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans. Bioresource Technology, 98(3), 534-538. https://doi.org/ 10.1016/j.biortech.2006.02.006
  • Cupp-Enyard, C. (2008). Sigma’s non-specific protease activity assay-casein as a substrate. JoVE (Journal of Visualized Experiments), (19), e899. https://doi.org/10.3791/899
  • Datta, S., Menon, G., Varughese, B. (2017). Production, characterization, and immobilization of partially purified surfactant–detergent and alkali-thermostable protease from newly isolated Aeromonas caviae. Preparative Biochemistry and Biotechnology, 47(4), 349-356. https://doi.org/ 10.1080/10826068.2016.1244688
  • Denizci, A. A., Kazan, D., Abeln, E. C. A., Erarslan, A. (2004). Newly isolated Bacillus clausii GMBAE 42: an alkaline protease producer capable to grow under higly alkaline conditions. Journal of Applied Microbiology, 96(2), 320-327. https://doi.org/10.1046/j.1365-2672.2003.02153.x
  • Dhandapani, R., Vijayaragavan, R. (1994). Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4. World Journal of Microbiology and Biotechnology, 10, 33-35. https://doi.org/10.1007/BF00357559
  • Dorra, G., Ines, K., Imen, B. S., Laurent, C., Sana, A., Olfa, T., Pascal, C., Thierry, J., Ferid, L. (2018). Purification and characterization of a novel high molecular weight alkaline protease produced by an endophytic Bacillus halotolerans strain CT2. International Journal of Biological Macromolecules, 111, 342-351. https://doi.org/10.1016/ j.ijbiomac.2018.01.024
  • Elbanna, K., Ibrahim, I. M., Revol-Junelles, A. M. (2015). Purification and characterization of halo-alkali-thermophilic protease from Halobacterium sp. strain HP25 isolated from raw salt, Lake Qarun, Fayoum, Egypt. Extremophiles, 19, 763-774. https://doi.org/10.1007/s00792-015-0752-3
  • Esakkiraj, P., Immanuel, G., Sowmya, S. M., Iyapparaj, P., Palavesam, A. (2009). Evaluation of protease-producing ability of fish gut isolate Bacillus cereus for aqua feed. Food and Bioprocess Technology, 2, 383-390. https://doi.org/10.1007/ s11947-007-0046-6
  • Farooq, S., Nazir, R., Ganai, S. A., Ganai, B. A. (2021). Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil. Scientific Reports, 11(1), 12768. https://doi.org/10.1038/ s41598-021-92197-w
  • Fujiwara, N., Masui, A., Imanaka, T. (1993). Purification and properties of the highly thermostable alkaline protease from an alkaliphilic and thermophilic Bacillus sp. Journal of Biotechnology, 30(2), 245-256. https://doi.org/ 10.1016/0168-1656(93)90117-6
  • Gençkal, H., Tari, C. (2006). Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats. Enzyme and Microbial Technology, 39(4), 703-710. https://doi.org/ 10.1016/j.enzmictec.2005.12.004
  • Hammami, A., Hamdi, M., Abdelhedi, O., Jridi, M., Nasri, M., Bayoudh, A. (2017). Surfactant-and oxidant-stable alkaline proteases from Bacillus invictae: characterization and potential applications in chitin extraction and as a detergent additive. International Journal of Biological Macromolecules, 96, 272-281. https://doi.org/ 10.1016/j.ijbiomac.2016.12.035
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There are 57 citations in total.

Details

Primary Language English
Subjects Food Biotechnology
Journal Section Articles
Authors

Bilge Sayın Börekçi 0000-0002-1898-0428

Sedat Dönmez This is me 0000-0002-9616-9841

Ayşe Avcı 0000-0001-7102-397X

Publication Date
Submission Date January 28, 2024
Acceptance Date April 6, 2024
Published in Issue Year 2024 Volume: 49 Issue: 3

Cite

APA Sayın Börekçi, B., Dönmez, S., & Avcı, A. (n.d.). PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM. Gıda, 49(3), 466-477. https://doi.org/10.15237/gida.GD24017
AMA Sayın Börekçi B, Dönmez S, Avcı A. PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM. The Journal of Food. 49(3):466-477. doi:10.15237/gida.GD24017
Chicago Sayın Börekçi, Bilge, Sedat Dönmez, and Ayşe Avcı. “PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM”. Gıda 49, no. 3 n.d.: 466-77. https://doi.org/10.15237/gida.GD24017.
EndNote Sayın Börekçi B, Dönmez S, Avcı A PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM. Gıda 49 3 466–477.
IEEE B. Sayın Börekçi, S. Dönmez, and A. Avcı, “PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM”, The Journal of Food, vol. 49, no. 3, pp. 466–477, doi: 10.15237/gida.GD24017.
ISNAD Sayın Börekçi, Bilge et al. “PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM”. Gıda 49/3 (n.d.), 466-477. https://doi.org/10.15237/gida.GD24017.
JAMA Sayın Börekçi B, Dönmez S, Avcı A. PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM. The Journal of Food.;49:466–477.
MLA Sayın Börekçi, Bilge et al. “PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM”. Gıda, vol. 49, no. 3, pp. 466-77, doi:10.15237/gida.GD24017.
Vancouver Sayın Börekçi B, Dönmez S, Avcı A. PRODUCTION OF ALKALINE PROTEASE BY A NOVEL ANAEROBIC BACTERIUM ISOLATED FROM A MUNICIPAL ANAEROBIC TREATMENT SYSTEM. The Journal of Food. 49(3):466-77.
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